Advanced Light Microscopy Unit

Overview

The Advanced Light Microscopy Unit (ALMU) is a walk-in core unit on a mission to help, support and educate the research community from CRG and beyond to do quantitative bioimaging. We provide support on a range of technologies starting from experimental design, sample preparation, acquisition, bioimage analysis and visualization.

Our unit consists of microscope systems specifically designed for fluorescence microscopy ranging from widefield, and confocal to super-resolution systems and helps reveal structures from macro to the nanoscale. Together with our next-door neighbors, the Mesoscopic Imaging Facility (MIF) of the EMBL Barcelona, we cover the full spectrum of light microscopy and we collaborate to assist the scientific community in advancing their research. Click on the services tab to learn more about our support and how to gain access to our instruments.

You can learn more about the unit by taking the virtual tour HERE.

Latest Updates

 

New Spinning disk confocal microscope with FRAP & super-resolution capabilities at ALMU We are delighted to offer the Evident/Olypmus IXplore SpinSR spinning disk confocal microscope as part of our portfolio. We've named it "SpinSR Live" to reflect its purpose. The SpinSR Live is equipped with 6 laser lines (405, 445, 488, 514, 561 and 640 nm), a 30x/1.05 NA and a 60x/1.3 NA silicon objective for live-cell imaging, 2 Hamamatsu Fusion BT CMOS cameras, and the SoRa system. Additionally, it includes a FRAP unit with 405, 488, and 640 nm lasers, and a full environmental chamber for temperature, CO2 and humidity control.   For more information, training on the system, or testing, please send us an Agnedo request or contact ALMU (almu@core.crg.eu).
	True Image Deconvolution, Restoration, and Analysis Workshop (13/03 & 03/04/2025) Join us for the upcoming True Image Deconvolution, Restoration, and Analysis workshop run by the Scientific Volume Imaging (SVI). This course will focus on obtaining high-quality microscopy images for reliable analysis results. Topics covered will include diffraction, acquisition pitfalls, spherical aberration, photon noise, Point Spread Function, Nyquist-Shannon Sampling Rate, Image Quality Control, crosstalk, (colocalization) analysis, and deconvolution. For more information follow the link here. Deadline for registration March 10, 2025.
	Lattice SIM 3 Launch Tour starting at ALMU (22-30/04/2024) ALMU is collaborating with Carl ZEISS Iberia to host the new Lattice SIM 3 system first landing in Spain. The system is designed to meet the imaging requirements of multicellular organisms and tissue sections. To register follow the link here. April 29: Virtual seminar covering the Lattice SIM 3 system capabilities and highlighting the technology. Followed by a virtual live demo of the system. April 29-30: Hands-on demo with researcher's own samples.
	Quantitative Light Microscopy Course - QLM2024 (22-26/04/2024) ALMU together with the CRG training team is organizing the second edition of the Quantitative Light Microscopy Course, QLM2024. This unique course covers the entire spectrum of bioimaging, starting from the fundamental basis of optical microscopy, instrumentation, specimen preparation, molecular markers, right up to the most recent and revolutionary development in bioimaging and bioimage data analysis, including artificial intelligence in microscopy along with hands-on practical sessions. For more information follow the link here.
ALMU welcomes a new staff member Sanni Erämies (15/01/2024) Join us in welcoming Sanni Erämies to the team! Sanni steps in during the one-year leave of Arrate Mallabiabarrena Ormaechea. With Sanni's previous experience as an imaging scientist at the Tampere Imaging Facility, Uni. of Tampere, we're excited to work with her and for the contributions she'll bring to ALMU.
	The Kingdome of Spain has joined Euro-Bioimaging (14/12/2023)  ALMU became part of two Spanish nodes for Euro-BioImaging: SLN@BCN - Barcelona Super Resolution Light Nanoscopy - Super Resolution Node, and Barcelona Live and Intravital - Advanced Light Microscopy Node (BLivIN). For more information follow the link here.
	OMERO Workshop (7-8/11/2024) ALMU together with OME, the University of Dundee, and CRG's SIT & CRG training are organizing the OMERO workshop. This course will give a solid ground for CRG researchers and all researchers who would like to use the open-source image data management software to enable access, analysis, visualization, sharing, and publishing of microscopy data. This course is also an important milestone for CRG to start using CRG's OMERO server for image data management. For more information follow the link here.
	Spotlight on Jeremy Vincencio, Shoma Nakagawa & ALMU in Evident's innovative laser scanning confocal system brochure We are proud to see the fruit of our successful collaboration with Evident and ALMU users: Jeremy Vincencio's (Stroustrup Lab) and Shoma Nakagawa (Cosma Lab) in the brochure of Evident's new innovative laser scanning confocal system FLUOVIEW FV4000. Tests on the prototype were carried out at ALMU. For more information follow the link here.
	ALMU collaborates with a global initiative to promote reproducible microscopy data publishing (21/09/2023)
	ALMU collaborates to reveal the ancient origins of neurons (19/09/2023)

ALMU collaborates to test the new function Dynamic Profiler from Carl Zeiss 
We are proud to see the successful launch of the new function Dynamic Profiler which enables the measurement of molecular dynamics with spatial information. Tests were carried out at ALMU, with ALMU and ALMU users. For more information follow the link here.

Elyra 7 with Lattice SIM2 workshop with Carl Zeiss (27/03/2023)
ALMU hosted the Elyra 7 workshop that presents the latest advances allowing gentle imaging of cellular structure in the nanoscale, using SIM, PALM/STORM technologies at high speed with the ability to image deeper into the samples.  

February 27: Virtual seminar covering the ELyra 7 system capabilities and highlighting the super-resolution technology. Followed by a virtual live demo of the system.
February 27 - March 3: Hands-on demo with researcher's own samples.

Quantitative Light Microscopy Course (20-24/02/2023) ALMU together with the CRG training team is organizing the very first Quantitative LightMicroscopy Course. This unique course covers the entire spectrum of bioimaging, starting from the fundamental basis of optical microscopy, instrumentation, specimen preparation, molecular markers, right up to the most recent and revolutionary development in bioimaging and bioimage data analysis, including artificial intelligence in microscopy. For more information follow the link here.

IXplore SpinSR workshop with Evident/Olympus (04/05/2022)
ALMU hosted the IXplore SpinSR workshop that presented the latest advances in multi-modal confocal spinning disk imaging of live cells and tissues in super-resolution mode.
May 4: Virtual seminar covering the IXplore SpinSR system capabilities and highlighting the super-resolution technology. Followed by a virtual live demo of the system.
May 5 - May 18: Hands-on demo with researcher’s own samples.

CRG at the Dubai Expo Spain Pavilion (03/2022)
Nadia Halidi has joined forces with Neoalgae, an Austrian biotechnology company, and The Others TV, an animation studio based in Barcelona to showcase the potential of microalgae as a sustainable future solution. The movies were acquired with a high-speed camera using darkfield microscopy with components provided specifically for this project by Carl Zeiss Iberia S.L. The production was showcased in the Spain Pavilion at the Expo 2020 Dubai from October 2021 to March 2022.

Nadia Halidi CV

February 2021  Head of the Advanced Light Microscopy Unit, CRG, Barcelona, Spain.
2018 - 2021      Assistant manager, then manager of Micron Oxford Advanced Bioimaging Unit, University of Oxford, Oxford, UK. 
2017 - 2018      Postdoctoral researcher and imaging facility manager at the Botnar Research Center, University of Oxford, Oxford, UK.
2011 - 2015      Postdoctoral fellow at the Dept. of Cell Biology, Harvard Medical School, Boston, MA, USA.
2007 - 2011      PhD in Biophysics, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.  
2005                    Teaching assistant at the Kalamazoo College, Kalamazoo, MI, USA.
2002 - 2006      Research and teaching assistant at the Dept. of Biophysics, University of Pécs, Pécs, Hungary.