Advanced Light Microscopy Unit
Advanced Light Microscopy Unit
Overview
It is the aim of this unit to provide instruments covering the whole application spectrum of advanced light microscopy and to make them available to researchers from the CRG as well as for visitors from other research institutions.
As Core Facility for light microscopy, the unit provides a number of advanced light microscopy systems, equipment for sample preparation and maintenance prior to imaging and resources for the subsequent processing of the image data.
The staff assists researchers in the experimental planning of light microscopy experiments and provides the in-depth training for the operation of the microscopes and for specific imaging techniques. Additionally, support is provided in the processing, rendering and analysis of the acquired datasets. If needed, custom analysis routines will be designed.
Nadia Halidi CV
February 2021 Head of the Advanced Light Microscopy Unit, CRG, Barcelona, Spain.
2018 - 2021 Assistant manager, then manager of Micron Oxford Advanced Bioimaging Unit, University of Oxford, Oxford, UK.
2017 - 2018 Postdoctoral researcher and imaging facility manager at the Botnar Research Center, University of Oxford, Oxford, UK.
2011 - 2015 Postdoctoral fellow at the Dept. of Cell Biology, Harvard Medical School, Boston, MA, USA.
2007 - 2011 PhD in Biophysics, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.
2005 Teaching assistant at the Kalamazoo College, Kalamazoo, MI, USA.
2002 - 2006 Research and teaching assistant at the Dept. of Biophysics, University of Pécs, Pécs, Hungary.
Services
- Consultation and assistance starting from experimental design, sample preparation, imaging, and data analysis
- Technology training on the operation of the unit’s microscope systems
- Support for experiments requiring advanced imaging methods
- User access to state-of-the-art microscopy systems
- User access to analysis workstations
- Image data analysis services
How to access / Instrument Booking
The Advanced Light Microscopy Unit (ALMU) is open to the research community from within CRG, the PRBB, and beyond. ALMU houses a range of state-of-the-art microscopes, from widefield, confocal to super-resolution systems. We offer training, instrumentation, and support for experimental design, sample preparation, data analysis, and visualization to the highest standards.
To request access and training, please read our Access Policy. Due to the high demand and to ensure that the training goes smoothly, please contact us 1-2 weeks in advance.
For existing users, all our resources can be booked through our booking system AGENDO: http://crg.agendo.science
ALMU Access Policy
ALMU COVID guidelines
Service Prices
Equipment
The equipment offer is available to trained users and covers all current imaging applications with high-end imaging systems.
Super-resolution microscopy
- Leica confocal STED 3X FALCON system with white light laser (WLL) and three depletion wavelengths (WLL: 470 - 670 nm, 592nm CW, 660nm CW, 775nm pulsed)
- Nikon N-STORM (405nm, 488nm, 561nm, 647 nm lasers)
- Zeiss LSM 980 with airyscan 2 (405, 488, 561, 639 nm lasers)
- Leica confocal CW STED super-resolution microscope with 592 nm CW depletion wavelength
- Leica Ground State Depletion (GSD) microscope system (488 nm, 532 nm, 647 nm, 405 nm backpumping)
Multiphoton microscopy
- Leica inverted confocal/multiphoton system (405, Argon ion, 561, 633nm and Mai Tai DeepSee)
- Leica upright confocal/multiphoton system (405, Argon ion, 543, 633nm and Mai Tai DeepSee)
Confocal Microscopy
- Leica STELLAIRS confocal system with white light laser ( WLL: 485 - 685, also 405, 488, 561 nm lasers)
- Leica inverted confocal system (405, 458,476,488,496,514,561 and 633 nm lasers)
- Leica inverted confocal system for live-cell imaging (458,476,488,514,543 and 633 nm lasers)
- Andor Dragonfly Spinning Disk confocal system (Borealis illumination, 488 and 561 nm lasers)
- Andor Revolution XD Spinning Disk confocal microscope (405, 445, 488, 514, 561 nm lasers)
Widefield Microscopy
- Zeiss Cell Observer fluorescence microscope for live-cell imaging
- Olympus MVX10 Macro Zoom fluorescence microscope
Screening Microscopy
- Molecular Devices ImageXpress Micro Purchase year: 2009
Computer resources
- AQUIFER HIVE for image processing and analysis
- 2 workstations for image processing
Workstations and Software packages
- Analysis workstations and AQUIFER HIVE for image processing and analysis
- Open source software: ImageJ/Fiji, Cel Profiler, Advanced Cell Classifier
- SVI Huygens Deconvolution software
- Bitplane Imaris 3D and 4D imaging software
Head of unit
