Advanced Light Microscopy Unit
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Advanced Light Microscopy Unit
Overview
It is the aim of this unit to provide instruments covering the whole application spectrum of advanced light microscopy and to make them available to researchers from the CRG as well as for visitors from other research institutions.
As Core Facility for light microscopy, the unit provides a number of advanced light microscopy systems, equipment for sample preparation and maintenance prior to imaging and resources for the subsequent processing of the image data.
The staff assists researchers in the experimental planning of light microscopy experiments and provides the in-depth training for the operation of the microscopes and for specific imaging techniques. Additionally, support is provided in the processing, rendering and analysis of the acquired datasets. If needed, custom analysis routines will be designed.
The Head of the Service is Timo Zimmermann. For any further information, please click here.
Latest Updates
New purchases:
- Installation of an Andor Dragonfly Spinning Disk confocal scanner on a Nikon Eclipse Ti2 microscope: The new system aims to extend the offer for fast and sensitive in-vivo confocal imaging that is needed by the unit users and will because of its modular design and excellent illumination optics (Borealis) serve as a platform for future technology upgrades. (9/2017)
- Acquisition of a Nikon N-STORM 4.0 system for localization-based super-resolution microscopy: This system extends the existing offer of super-resolution systems in the unit by adding the capability to execute Stochastic Optical Reconstruction Microscopy (STORM) based on activator-emitter pairs. (9/2017)
- Purchase of a Leica 93x 1.3 NA Glycerol objective with motorized correction collar for STED imaging on the Leica STED 3X super-resolution system: This new objective allows to efficiently use Stimulate Emission Depletion (STED) microscopy in thick samples and in living samples that would be mismatched in the refractive index to STED objectives with oil immersion. (5/2017)
Timo Zimmermann CV
2000 PhD in Biology at the Ludwig-Maximilians-University, Munich
2000 EMBO Short Term Fellowship at the European Molecular Biology Laboratory, Heidelberg
2001-2006 Senior Technical Officer at the Advanced Light Microscopy Facility, EMBL, Heidelberg
2007- Head of the Advanced Light Microscopy Unit at the Centre for Genomic Regulation, Barcelona
How to access / Instrument Booking
CRG members can access the CRG intranet webpage with their username and password and go directly to the Microscope Reservation Page.
Other PRBB members and external users need to fill this form (attached document) and bring it to the ALMU office. A username and password will be sent in order to access the microscope reservation page.
Contact facility staff for an introductory session in the microscopes and in case of need of assistance with the experiment preparation and image analysis.
Service Prices
Services
- Technology training on the operation of the unit’s microscope systems
- Support for experiments requiring advanced imaging methods
- User access to high-end light microscopy systems covering all currently existing imaging fields
Equipment
The equipment offer is available to trained users and covers all current imaging applications with high-end imaging systems.
Super-resolution microscopy
- Leica TCS SP8 STED 3X super-resolution microscope with white light laser and three depletion wavelengths (592nm CW, 660nm CW, 775nm pulsed) for 3D STED Purchase year: 2015
- Leica TCS SP5II CW STED super-resolution microscope with 592 nm CW depletion and two HyD detectors Purchase year: 2010
- Leica Ground State Depletion (GSD) microscope system for localization-based super-resolution microscopy (488 nm, 532 nm, 647 nm, 405 nm backpumping) Purchase year: 2012
- Nikon N-STORM 4.0 microscope system for localization-based super-resolution microscopy (405nm, 488nm, 561nm, 647 nm) Purchase year: 2017
Multiphoton microscopy
- SpectraPhysics MaiTai DeepSee tunable pulsed infrared laser Purchase year: 2009, upgrade 2012
- Leica TCS SP5 AOBS inverted confocal microscope (Purchase year: 2007) with Picoquant TCSPC hardware for FLIM and FCS Purchase year: 2009
- Leica TCS SP5 CFS (fixed stage) upright confocal microscope (Purchase year: 2008) with two non-descanned HyD detectors Purchase year: 2012
Confocal Microscopy
- Leica TCS SP8 AOBS inverted confocal microscope Purchase year: 2016
- 1 Leica TCS SP8 and 3 Leica TCS SP5 confocal microscopes (see super-resolution and multiphoton equipment)
- Leica TCS SPE inverted confocal microscope Purchase year 2007
- Andor Dragonfly Spinning Disk confocal module on a Nikon Eclipse Ti2 microscope Purchase year: 2017
- Andor Revolution XD Spinning Disk confocal microscope Purchase year: 2008
Widefield Microscopy
- Zeiss Cell Observer HS with Zen Blue software Purchase year: 2007
Screening Microscopy
- Molecular Devices ImageXpress Micro Purchase year: 2009
Computer resources
- Acquifer HIVE for intermediate storage and image processing Purchase year: 2015
- 2 workstations for image processing
Software packages
- SVI Huygens Deconvolution software
- Bitplane Imaris 3D and 4D imaging software
- Open Source packages available on HIVE and the workstations: Fiji, Cell Profiler
Work Sheets
No resources available at the moment.
Head of unit
Technicians
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Technicians
UPF Unit Members
