Advanced Light Microscopy Unit

Advanced Light Microscopy UnitAdvanced Light Microscopy Unit

Advanced Light Microscopy Unit

Head of Unit

Advanced Light Microscopy Unit

Head of Unit
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Overview

It is the aim of this unit to provide instruments covering the whole application spectrum of advanced light microscopy and to make them available to researchers from the CRG as well as for visitors from other research institutions.

As Core Facility for light microscopy, the unit provides a number of advanced light microscopy systems, equipment for sample preparation and maintenance prior to imaging and resources for the subsequent processing of the image data.

The staff assists researchers in the experimental planning of light microscopy experiments and provides the in-depth training for the operation of the microscopes and for specific imaging techniques. Additionally, support is provided in the processing, rendering and analysis of the acquired datasets. If needed, custom analysis routines will be designed.

Nadia Halidi CV

February 2021  Head of the Advanced Light Microscopy Unit, CRG, Barcelona, Spain.
2018 - 2021      Assistant manager, then manager of Micron Oxford Advanced Bioimaging Unit, University of Oxford, Oxford, UK. 
2017 - 2018      Postdoctoral researcher and imaging facility manager at the Botnar Research Center, University of Oxford, Oxford, UK.
2011 - 2015      Postdoctoral fellow at the Dept. of Cell Biology, Harvard Medical School, Boston, MA, USA.
2007 - 2011      PhD in Biophysics, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.  
2005                    Teaching assistant at the Kalamazoo College, Kalamazoo, MI, USA.
2002 - 2006      Research and teaching assistant at the Dept. of Biophysics, University of Pécs, Pécs, Hungary.

Equipment

The equipment offer is available to trained users and covers all current imaging applications with high-end imaging systems.

Super-resolution microscopy

  • Leica confocal STED 3X FLIM system with white light laser and three depletion wavelengths (592nm CW, 660nm CW, 775nm pulsed)
  • Zeiss LSM 980 with airyscan 2 (405, 488, 561 and 639 nm lasers)
  • Leica confocal CW STED super-resolution microscope with 592 nm CW depletion wavelength
  • Leica Ground State Depletion (GSD) microscope system (488 nm, 532 nm, 647 nm, 405 nm backpumping)
  • Nikon N-STORM (405nm, 488nm, 561nm, 647 nm lasers)

Multiphoton microscopy

  • Leica inverted confocal/multiphoton system (405, Argon ion, 561, 633nm and Mai Tai DeepSee)
  • Leica upright confocal/multiphoton system (405, Argon ion, 543, 633nm and Mai Tai DeepSee)

Confocal Microscopy

  • Leica inverted confocal system (405, 458,476,488,496,514,561 and 633 nm lasers)
  • Leica inverted confocal system for live-cell imaging (458,476,488,514,543 and 633 nm lasers)
  • Andor Dragonfly Spinning Disk confocal system (Borealis illumination, 488 and 561 nm lasers)
  • Andor Revolution XD Spinning Disk confocal microscope (405, 445, 488, 514, 561 nm lasers)

Widefield Microscopy

  • Zeiss Cell Observer fluorescence microscope for live-cell imaging
  • Olympus MVX10 Macro Zoom fluorescence microscope

Screening Microscopy

  • Molecular Devices ImageXpress Micro Purchase year: 2009

Computer resources

  • AQUIFER HIVE for image processing and analysis
  • 2 workstations for image processing

Workstations and Software packages

  • Analysis workstations and AQUIFER HIVE for image processing and analysis
  • Open source software: ImageJ/Fiji, Cel Profiler, Advanced Cell Classifier
  • SVI Huygens Deconvolution software
  • Bitplane Imaris 3D and 4D imaging software