Role of PADI2-mediated citrullination of the Pol-II CTD in transcription elongation
Among the PADI family of protein arginine de-iminases, T47D cells only express PADI2 and PADI3. In response to hormone PADI2 is needed for hormonal gene regulation, interacts with PR and citrullinates R1810 in Pol-II CTD repeat 31. A Pol-II R1810K mutant Pol-II is defective in promoter release. We are exploring how hormones control PADI2 activity and genome location.