Protein Technologies Unit

How to access

All services offered by the Protein Technologies Unit are accessible to all the scientific community. Access is always open and services are performed upon request.

All services and equipment offered by the Protein Technologies Unit are accessible by the web platform AGENDO.
You can access to AGENDO in the following link: https://crg.agendoscience.com/

Service Prices

Prices

Services & Applications

CRG reagent collection: 

• More than 20k reagents including compound libraries, antibodies, vectors, cells, DNA assembly mix for Gibson cloning and several proteins for multiple applications 

DNA assembly: 

• Mutagenesis; Vector reconstruction: e.g Crispr HDR vector for KI applications; HTP cloning vector preparation

Protein expression: 

• Small and large scale (up to 6L round) protein expression and solubility screening assay in bacteria, mammalian cells (EXPI293 and EXPICHO) and insect cells (sf21 and Hi5); 

Protein purification and QC: 

• Automatized 2-3 step protein purification (Akta FPLCs);
• Customized protein purification pipeline.
• Recombinant antibody/nanobody purification
• Protein labelling
• Sample endotoxin analysis using Endosafe® nexgen-PTS

Biomolecular interaction analysis: 

• Kinetic binding: ka, kd
• Equilibrium binding: KD
• Macromolecular and small molecule binding
• Affinity measurements in complex mixtures (cell lysates, serum, detergents, liposomes)
• Bioluminescence complementation assay (HTP nanoBRET assay)

Biomolecular oligomerization/stability analysis: 

• Measures absolute molecular weight, size, and conformation of macromolecules in solution. Analyzes the absolute molar mass and size of macromolecules in solution.
• Measures particle size distributions and diffusion coefficients.

Cross technologies services

Gene knockout and knock-in cell engineering with Tissue Engineering Unit: 

• The Protein Technologies Unit together with the Tissue Engineering Unit is providing a fully integrated CRISPR technology service. Our Unit is involved in experimental design, reagent preparation and data analysis while the tissue engineering facility is involved in cell manipulation. 

Metagenomic project service with the Genomic and Bioinformatic Unit: 

• The Protein Technologies Unit is providing expertise and capacity in automation of DNA extraction on 96 well plates from biological samples creating a fully integrated pipeline including sequencing and data analysis produced from the other 2 Units. 

Protein Characterization service  with the Proteomic Unit:

• The Protein Technologies Unit is providing Intact protein analysis (MW) of purified proteins and antigen characterization of antibodies/nanobodies (together with Proteomic Unit)

Equipment

Protein purification  and QC platform: 

• 1 AKTAvant (Cytiva, 2018) and an AKTA pure (Cytiva, 2019): FPLC priority for the Unit  for purification of biomolecules
• 1 AKTA Pure (Cytiva, 2020): FPLC shared with the CRG users
• Endosafe® nexgen-PTS for sample endotoxin analysis 

Biophysical  characterization platform: 

• Biacore T100 SPR instrument (Cytiva, 2009) with an upgrade on T200 (2023) for the study of kinetics and affinity of biomolecular interaction
• Monolith NT.115 microscale thermophoresis instrument (Nanotemper, 2013) to measure molecular interactions.
• SEC-MALS system (2016): CBM-20A series HPLC system (Shimadzu), DAWN HELEOS II and a T-rEx (Wyatt) for the measurement of absolute molecular weight of biomolecules.
• Prometheus Panta (Nanotemper, 2024): to provide multi-parameter stability characterization by collecting data on thermal unfolding, particle sizing, and aggregation simultaneously, throughout the entire thermal ramp, for high-resolution, domain-specific stability characterization of biologics.
• TwoMP mass photometer (Refyen, 2023): for the precise measurement of the mass of individual molecules in solution.

Others: 

• Tecan liquid handling robotic platform Evo200 (Tecan, 2010) for automatization of large scale and high throughput applications.
• Revity Operetta microscope (2008) for high content screening