This course will focus on the next generation sequencing library preparation for sequencing on the Illumina platform. Aside from library preparation for standard genomic sequencing and for directional mRNA seq, this course will teach advanced methods for the preparation of libraries from very small quantities of starting material. In particular, the course schedule will include teaching smart seq, a protocol for sequencing the transcriptomes of single cells and explain MALBAC, designed to sequence single cell genomes.

14/07/201412:00MARIE CURIEPRBB-CRG SessionsJorge MoscatCell Death and Survival Networks Program, NCI-Designated Cancer Center, Sanford-Burnham Medical Research Institute, La Jolla (CA) US“New role of metabolic reprograming in stroma-induced tumorigenesis”Host: Antonio García de los Herreros

11/07/201410:00RAMÓN Y CAJALGene Regulation,Stem Cells&Cancer Progr.Kyle LohInstitute for Regenerative Biology, Stanford Medical School, Stanford University, Stanford (CA) US"Deconstructing developmental biology: efficient pluripotent stem cell differentiation by logically guiding lineage bifurcations"Host: Graf, Thomas

10/07/201412:30MARIE CURIECore Facilities SeminarsMartí BernardoEMBL-EBI, Cambridge, UKModelling phosphoproteomics to characterize signalling deregulation in multiple sclerosisHost: Sabido Aguade, Eduard

10/07/201411:00R_473.10_AULAPRBB Computational Genomics SeminarsDidac SantesmassesComputational Biology of RNA Processing, CRG"Selenoprotein extinctions in insects"Host: Garrido Enamorado, Romina

09/07/201411:00R_470_SEM2Cell and Developmental Biology ProgrammeSebastian Baumann (Postdoc Candidate Seminar)Prof. Michael Feldbrügge, Institute for Microbiology. Heinrich Heine Universtity Düsseldorf."Endosomal transport of septin mRNA and protein indicates local translation on endosomes"Host: Carbonell García, Rut

08/07/201412:30CHARLES DARWINCore Facilities SeminarsAlexander LeitnerETH, Zurich CHAnalysis of protein complexes by chemical cross-linking/mass spectrometry: A complementary chemistry and structural analysis of mitochondrial ribosomeHost: Sabido Aguade, Eduard