PRBB-CRG Sessions Job Dekker

16/04/202614:00MARIE CURIEPRBB-CRG SessionsJob DekkerUMass Chan Medical School"A pathway for formation of an RNA-mediated nuclear compartment"Host: Marc Marti-RenomAbstract:Mammalian nuclei are spatially compartmentalized so that active and inactive segments of the genome occupy separate sub-nuclear neighborhoods. Compartments are often found in association with nuclear structures such as the nuclear lamina, nucleoli, and nuclear speckles (speckles), suggesting links between them. The mechanisms by which compartments form remain largely unknown. Speckles are large nuclear bodies that contain high concentrations of RNA splicing factors and associated chromatin has a high density of highly expressed genes. Combining liquid chromatin Hi-C, immunofluorescence, fluorescence in situ hybridization, live cell imaging, nascent transcript analysis, and polymer modeling we have determined the biophysical and molecular basis for maintenance of the speckle-associated chromosomal compartment. I will propose a pathway for formation of the speckle chromosomal compartment.