PRBB-CRG Sessions Kathryn Lilley

04/12/202512:00PRBB AuditoriumPRBB-CRG SessionsKathryn LilleySubcellular proteome and transcriptome, Department of Biochemistry, University of Cambridge"Unveiling the Subcellular Complexity: Beyond Protein Expression"Host: Eduard SabidóAbstract:Biological systems function through intricate regulatory networks that go far beyond simple changes in protein levels. These networks involve multiple layers of sophisticated multi-omics control, including non-coding RNA regulation, post-transcriptional and post-translational modifications, and dynamic subcellular localization. Many of these regulatory mechanisms rely on the dynamic interplay between proteins and RNA molecules. A comprehensive understanding of these multi-layered control systems is crucial for fully elucidating biological processes.
This presentation will emphasize the importance of studying how the proteome responds to cellular perturbations through spatial redistribution, showing that exclusive focus on protein abundance changes can lead researchers to overlook critical cellular responses¹.
I will explore how RNA-protein interactions undergo dynamic shifts during key cellular transitions²,³. Additionally, I will present a novel methodology that enables simultaneous mapping of both protein spatial distribution and RNA transcript localization throughout entire cells4. I will demonstrate how this approach has revealed the coordinated translocation of RNA and proteins during unfolded protein response (UPR) activation, providing new insights into RNA trafficking to stress granules under cellular stress conditions.