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Courses@CRG: Biological Super-Resolution Microscopy

Courses@CRG: Biological Super-Resolution MicroscopyCourses@CRG: Biological Super-Resolution Microscopy

02/05/2016 05/05/2016
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Courses@CRG: Biological Super-Resolution Microscopy

In the last years, super-resolution light microscopy equipment has become more widely available for biological researchers. Most if not all of the existing approaches do however present challenges in sample preparation, image acquisition and also the subsequent analysis that need to be met for the successful execution of a biological experiment. The course will provide an introduction into super-resolution imaging for STED and localization microscopy on state of the art instrumentation and focus on the important aspects of label selection, labeling strategies, sample mounting, imaging (3D) and subsequent anaylsis of STED and dSTORM datasets.

Technologies and methods:

  • Labeling considerations, imaging buffers and mounting media for super-resolution microscopy: localization microscopy and STED
  • Stimulated emission depletion (STED) microscopy
    • Pulsed, CW and time-gated STED imaging
    • Multichannel STED imaging
    • 3D STED imaging
    • in vivo STED imaging
  • Localization based super-resolution microscopy methods
    • Multichannel 3D direct Stochastic Optical Reconstruction Microscopy (dSTORM)
    • Point Accumulation for Imaging in Nanoscale Topography (PAINT)
  • Rendering and processing of super-resolution datasets
  • Deconvolution of 3D STED datasets
  • Rendering of point-based SR data
  • Registration of image channels
  • Adaptive Optics in SR microscopy
Timo Zimmermann   CRG, Barcelona
Kees Jalink   Netherlands Cancer Institute, Amsterdam
Lorenzo Albertazzi    IBEC, Barcelona
Magali Mondin   Institite of Biology Valrose, Nice
Raquel García   CRG, Barcelona
Arrate Mallabiabarrena   CRG, Barcelona
Xavier Sanjuan   UPF, Spain
Nathalie Garin   Leica Microsystems


Registration :
Only online registration is accepted
Number of participants : max. 16
Registration fee: 400€

Registration fee includes participation in workshop, all necessary tools to follow the course, didactic material, coffee breaks and lunches.
Worldwide participants will be selected according to their CV and Motivation Letter submitted.

2 Registration Fee Waivers to help lower-income students and facilitate the participation of those coming from labs located in countries in need of scientific strengthening will be offer.

CRG – Centre for Genomic Regulation
Dr. Aiguader 88
08003 Barcelona (Spain)
How to get to the CRG ?

Sharon Bel
CRG – Centre for Genomic Regulation
Phone + 34 93 316 0375

Supported by:
We acknowledge support from the Spanish Ministry of Economy and Competitiveness,
"Centro de Excelencia Severo Ochoa 2013-2017", SEV 2012-0208.