Decoding Transcriptomes: a practical course on RNAseq 2027

This hands-on program provides a comprehensive introduction to RNA sequencing, guiding participants through the complete workflow from sample preparation to data analysis. Participants will gain practical experience in RNA quality control, mRNA library preparation for Next Generation Sequencing (NGS), and library quality control. On the computational side, they will work with real sequencing data to learn key steps including data quality assessment, alignment, quantification, differential expression analysis, and functional interpretation.
 
The course integrates laboratory practice with computational training, equipping participants with the skills needed to design robust RNA-seq experiments and to apply reproducible analysis workflows following the FAIR practices. The program is complemented by theoretical lectures on state-of-the-art transcriptomics and bioinformatics, a guided visit to the CRG Genomics Unit, and networking activities.