Advanced Light Microscopy Unit

Advanced Light Microscopy Unit

Advanced Light Microscopy Unit

Head of unit
j

Advanced Light Microscopy Unit

Head of unit
j

Overview

It is the aim of this unit to provide instruments covering the whole application spectrum of advanced light microscopy and to make them available to researchers from the CRG as well as for visitors from other research institutions.

As Core Facility for light microscopy, the unit provides a number of advanced light microscopy systems, equipment for sample preparation and maintenance prior to imaging and resources for the subsequent processing of the image data.

The staff assists researchers in the experimental planning of light microscopy experiments and provides the in-depth training for the operation of the microscopes and for specific imaging techniques. Additionally, support is provided in the processing, rendering and analysis of the acquired datasets. If needed, custom analysis routines will be designed.

The Head of the Service is Timo Zimmermann. For any  further information, please click here.

Latest Updates

New purchases:

  • Installation of an Andor Dragonfly Spinning Disk confocal scanner on a Nikon Eclipse Ti2 microscope: The new system aims to extend the offer for fast and sensitive in-vivo confocal imaging that is needed by the unit users and will because of its modular design and excellent illumination optics (Borealis) serve as a platform for  future technology upgrades. (9/2017)
  • Acquisition of a Nikon N-STORM 4.0 system for localization-based super-resolution microscopy: This system extends the existing offer of super-resolution systems in the unit by adding the capability to execute Stochastic Optical Reconstruction Microscopy (STORM) based on activator-emitter pairs. (9/2017)
  • Purchase of a Leica 93x 1.3 NA Glycerol objective with motorized correction collar for STED imaging on the Leica STED 3X super-resolution system: This new objective allows to efficiently use Stimulate Emission Depletion (STED) microscopy in thick samples and in living samples that would be mismatched in the refractive index to STED objectives with oil immersion. (5/2017)

Timo Zimmermann CV

2000 PhD in Biology at the Ludwig-Maximilians-University, Munich
2000 EMBO Short Term Fellowship at the European Molecular Biology Laboratory, Heidelberg
2001-2006 Senior Technical Officer at the Advanced Light Microscopy Facility, EMBL, Heidelberg
2007- Head of the Advanced Light Microscopy Unit at the Centre for Genomic Regulation, Barcelona

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