Biogenesis of Golgi to cell surface transport carriers
We have found that the formation of Golgi to cell surface transport carriers requires PKD , which is recruited to the trans Golgi network (TGN) by the membrane-associated diacylglycerol (DAG). PKCeta activates PKD at the TGN, and the activated PKD regulates the production of DAG by activating enzymes involved in its production and inhibiting those that consume it thereby increasing the local concentration of DAG The neutral phospholipid, DAG is the final component that destabilizes a domain of the cargo filled TGN to generate carriers that are transported to the cell surface. A genome wide screen was carried out to identify new components involved in protein secretion and Golgi organization. A number of novel components have been identified and called TANGO (for transport and Golgi organization). Work is in progress to reveal the mechanism of TANGO gene products in protein secretion and PKD dependent membrane fission.