CRG/UPF FACS Unit

CRG/UPF FACS Unit

CRG/UPF FACS Unit

Head of unit
j

CRG/UPF FACS Unit

Head of unit
j

Head of the CRG/UPF FACS Unit
UPF-University Pompeu Fabra
Room 360.01
Tel. +34 93 316 08 50

1996 PhD in Biochemistry at the Autonomous University of Barcelona (UAB), Barcelona, Spain.
1996-2000 Postdoc at Oncologic Research Institute (IRO), Barcelona, Spain.
2000-2001 PI at IDIBAPS, Barcelona, Spain.
2001-2010 Head of Flow Cytometry Core Facility at Pompeu Fabra University (UPF), Barcelona, Spain.
Since March 2010 Head of Flow Cytometry Core Facility at UPF/CRG, Barcelona, Spain.

Overview

Flow cytometry studies optical parameters emitted by particles (cells, cell fractions). Flow cytometers can study a series of parameters of individual particles simultaneously, quickly and on a large number of individualized particles in suspension. Flow cytometers use lasers as a source of light excitement; therefore, it must be possible to mark the particles with one or more fluorescent substances. Information is also collected regarding the size and structural complexity of each particle. This multiparameter study of each particle enables us to analyse subpopulations in complex samples through electronic sorting. Some cytometers are also equipped with sorting and collection modules for particles of interest.

The most important applications of flow cytometry include those relating to the study of cell surface receptors, nuclear and cytoplasmic antigens, DNA content, enzyme activity, cell integrity and membrane permeability and calcium flows.

The Unit currently hosts five analyzers and two sorters, and is therewith the largest Becton Dickinson site in Spain. The sorters are operated by the facility personnel, who also support the users in experimental design and data analysis. The analyzers are operated by the users themselves, after they have been trained. The facility personnel helps with the experimental design and supervises and monitors the experiments when necessary.

Available equipment:

Instrument Laser
Ex line (nm)
Parameters
FSC/SSC + FL
Application
 
FACScalibur 488, 633 2 + 4 analyzer
FACScanto 488, 633 2 + 6 analyzer
LSR II 488, 633, 355, 405 2 + 10 analyzer
LSR Fortessa 488, 640, 405, 561 2 + 13 analyzer
SONY SP6800 488, 407, 640 2 + 34 analyzer
FACSAria II SORP 488, 640, 355, 405, 561 2 + 18 cell sorter
BD Influx 488, 640, 355, 457, 561 2 + 14 cell sorter

For further information, please contact Òscar Fornas.