CRG/UPF FACS Unit
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Head of the CRG/UPF FACS Unit
UPF-University Pompeu Fabra
Tel. +34 93 316 08 50
1996 PhD in Biochemistry at the Autonomous University of Barcelona (UAB), Barcelona, Spain.
1996-2000 Postdoc at Oncologic Research Institute (IRO), Barcelona, Spain.
2000-2001 PI at IDIBAPS, Barcelona, Spain.
2001-2010 Head of Flow Cytometry Core Facility at Pompeu Fabra University (UPF), Barcelona, Spain.
Since March 2010 Head of Flow Cytometry Core Facility at UPF/CRG, Barcelona, Spain.
Flow cytometry studies optical parameters emitted by particles (cells, cell fractions). Flow cytometers can study a series of parameters of individual particles simultaneously, quickly and on a large number of individualized particles in suspension. Flow cytometers use lasers as a source of light excitement; therefore, it must be possible to mark the particles with one or more fluorescent substances. Information is also collected regarding the size and structural complexity of each particle. This multiparameter study of each particle enables us to analyse subpopulations in complex samples through electronic sorting. Some cytometers are also equipped with sorting and collection modules for particles of interest.
The most important applications of flow cytometry include those relating to the study of cell surface receptors, nuclear and cytoplasmic antigens, DNA content, enzyme activity, cell integrity and membrane permeability and calcium flows.
The Unit currently hosts five analyzers and two sorters, and is therewith the largest Becton Dickinson site in Spain. The sorters are operated by the facility personnel, who also support the users in experimental design and data analysis. The analyzers are operated by the users themselves, after they have been trained. The facility personnel helps with the experimental design and supervises and monitors the experiments when necessary.
Ex line (nm)
FSC/SSC + FL
|FACScalibur||488, 633||2 + 4||analyzer|
|FACScanto||488, 633||2 + 6||analyzer|
|LSR II||488, 633, 355, 405||2 + 10||analyzer|
|LSR Fortessa||488, 640, 405, 561||2 + 13||analyzer|
|SONY SP6800||488, 407, 640||2 + 34||analyzer|
|FACSAria II SORP||488, 640, 355, 405, 561||2 + 18||cell sorter|
|BD Influx||488, 640, 355, 457, 561||2 + 14||cell sorter|
For further information, please contact Òscar Fornas.