{"text_format":"1","workbook":"xlsx","n_sheets":2,"sheets":["1. Guidelines","2. Sequencing Request SAMPLES "],"not_rendered":{"charts":0,"images":3,"macros":0,"external_links":0,"defined_names":1,"comments":0,"pivot_tables":0},"unopened":[],"bounds":{"document_bytes":16777216,"sheet_cells":100000,"rows_first":2000,"rows_last":500,"rows_between":2000,"halvings":0,"cell_chars":16384,"cell_head_chars":4096},"cells_cut":0,"cells_raw":0,"read":"whole","date_system":"1900"}

{"sheet":"1. Guidelines","index":0,"n_rows":84,"n_cols":21,"rows_kept":84,"cut":false,"hidden":false,"merged":[[66,15,66,17],[9,1,9,18],[26,1,26,2],[28,1,28,2],[29,1,29,2],[30,1,30,2],[30,5,30,9],[29,3,29,4],[30,3,30,4],[31,1,31,2],[53,2,54,17],[33,1,34,17],[31,5,31,9],[26,5,26,9],[28,5,28,9],[29,5,29,9],[27,1,27,2],[27,3,27,4],[27,5,27,9],[26,3,26,4],[28,3,28,4],[31,3,31,4]]}
[]
[null,"HOW TO FILL SEQUENCING REQUEST SAMPLES"]
[]
[null,"Remember that you have to fill 1 Request form per application. "]
[null,"* Mandatory fields"]
[]
[null,"CONTACT and SUBPROJECT DETAILS"]
["*","Main User: User responsible for the samples and the project. Please specify full name and contact e-mail."]
["*","PI name: PI responsible for the project. Please specify full name and contact e-mail."]
[null,"Additional contact 1 or 2: Any person that needs to receive information about the project and the final data. Please specify full name and contact e-mail."]
["*","Center: your institution."]
[null,"Group name: your group name inside your institution."]
[null,"Experiment name: name that you give to your experiment."]
[null,"Official code project: example FIS-089932."]
["*","CRG Quotation addressed to (name & email): Person to whom Genomics Unit have to address the quotation, usually PI or other collaborator who will pay the project."]
["*","CRG Quotation associated nº: CRG quotation number for the requested service. If you have not requested it in advance and you do not have it, please, leave it in blank."]
["*","TENDER / Minor contract reference: If the request are part of a TENDER or Minor Contract, please specify the reference. "]
[]
[null,"APPLICATION"]
["*","Application: Please, select the desired application. If you need other application not listed, please specify it in the corresponding field and contact Genomincs Unit."]
[null,"For small RNAseq the standard selection is 18-32nt, if you need/want other sizes, please specify it in the corresponding field."]
[]
[null,"SEQUENCING DETAILS"]
[null,"NEW - New sequencer available at the Genomics Unit, NovaSeq 6000."]
["*","Million reads / sample: nº of millions reads that you need for each sample."]
["*","Sequencing format: Please, indicate your desire sequencing format. Here you have a brief list of sequencer specifications:"]
[null,"Sequencer",null,"Flowcell / kit type",null,"Million reads per flowcell (Expected) "]
[null,"NovaSeq6000",null,"S2",null,3000]
[null,"NextSeq2000",null,"P2",null,400]
[null,"NextSeq2000",null,"P3",null,1200]
[null,"NextSeq500",null,"Mid Output",null,130]
[null,"NextSeq500",null,"High Output",null,400]
[]
[null,"Other length: If you need a run with a sequencing format not listed in the request, is mandatory to fill a complete run, take into account specifications for each sequencer. After that, please indicate the sequencing format (cycles for Read1 - index i7 - index i5 - Read2)."]
[]
[null,"In case of any doubts, contact Genomics Unit."]
["*","Not for sequencing: You only need library preparation, not sequencing at the Unit."]
[null,"Select one option: Sequencing format or Not for sequencing"]
[]
[null,"OBSERVATIONS"]
[null,"Please, indicate any important/special issue with your project."]
[]
[null,"QUALITY CONTROL OF THE SAMPLES"]
["*","YES / NO: select one option. It refers to quality control of samples before library preparation, quality control of libraries is included in libray prep."]
[null,"YES: samples need to be quality checked at Genomics Unit."]
[null,"NO: samples NOT needed to be quality checked at Genomics Unit due to it has been done by user and/or agreeded with Genomics Unit. "]
[null,null,"Done by user: it is mandatory to fill the corresponding information in the table Sample Details and attach the results."]
[null,null,"Not QC needed: have to be previously agreeded with Genomics Unit, just for special subprojects / samples."]
[]
[null,"SAMPLE DETAILS"]
[null,"Plate name: If you want to send samples in plates, have to be previously agreed with Unit. These fields have restrictions, in case of error it is indicated in the next columns, please correct it."]
[null,null,"Well plate: if you have fill plate name, a well plate will appear at Sample Information table. Please, fill your plate in this order (by columns) and fill the sample information in the corresponding well."]
[null,"Sample Information"]
[null,"*","Sample Name: Use the same name as in the tube caps. Names should be alphanumerical, 6 characters maximum, no space, only accepted underscore. These fields have restrictions, in case of error it is indicated in the next columns, please correct it."]
[]
[null,"*","Type of sample: Select just one option."]
[null,"*","Sample Specie: Use NCBI Taxonomy name, not taxonomy ID. Example: Homo sapiens, not 9606."]
[null,"*","Volume (ul): Please be sure that volume is correct for each sample, this is very important to calculate the total amount of sample."]
[null,"**","Conc. (ng/ul): Mandatory ng/ul. Very important to take into account if you have measured your samples using a dilution, you have to apply de dilution factor to obtain the final concentration. Maximum 2 decimals."]
[null,"**","Nanodrop ratios: If you have measured your samples with nanodrop, please include this information."]
[null,"**","Quality Score (RIN / DIN): RNA or DNA Integrity number, usually given by instruments like Bioanalyzer, Fragment Analyzer or Tapestation."]
[null,null,"** These fields are mandatory if QC of samples is already done by user and it have been agreed with Genomics Unit of not to repeat them."]
[]
[null,"If you paste values from other files, please PASTE AS VALUES"]
[null,"Once all information is correctly filled without errors, you can send Sequencing Request form to ngs@crg.eu."]
[]
[null,"Example how to fill Sequencing Request (Samples Information)"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Correct Sample Name Format"]

{"sheet":"2. Sequencing Request SAMPLES ","index":1,"n_rows":184,"n_cols":49,"rows_kept":184,"cut":false,"hidden":false,"merged":[[17,5,17,6],[18,5,18,6],[78,3,78,5],[79,3,79,5],[40,2,56,7],[59,2,60,14],[20,4,20,6],[34,5,34,6],[20,11,20,12],[17,11,17,12],[19,11,19,12],[83,45,84,45],[83,13,85,13],[84,48,85,48],[83,44,84,44],[83,3,85,3],[83,4,85,4],[83,5,85,5],[83,12,85,12],[83,6,85,6],[83,8,85,8],[83,7,85,7],[83,17,85,17],[83,10,85,10],[83,11,85,11],[83,43,84,43],[83,36,84,41],[83,14,85,14],[83,9,85,9],[83,33,85,33],[83,15,85,15],[5,4,5,6],[13,4,13,6],[9,5,9,6],[15,11,15,13],[5,11,5,14],[7,11,7,14],[11,11,11,13],[9,11,9,13],[13,11,13,13],[7,4,7,6],[11,5,11,6],[15,4,15,6],[76,36,76,41],[71,17,72,17],[73,17,74,17],[75,17,76,17],[68,3,69,13],[73,2,74,14],[75,2,75,14]]}
[]
[null,null,"SEQUENCING REQUEST FORM (SAMPLES)",null,null,null,null,null,null,null,"GENOMICS UNIT - CRG Core Facilities"]
[]
[null,null,"CONTACT and SUBPROJECT DETAILS"]
[]
[null,null,"Main User Name:",null,null,null,null,null,null,"Main User contact e-mail: "]
[]
[null,null,"PI name:",null,null,null,null,null,null,"PI contact e-mail:"]
[]
[null,null,null,null,"Additional contact 1:",null,null,null,null,null,"Add. Contact 1 e-mail:"]
[]
[null,null,null,null,"Additional contact 2:",null,null,null,null,null,"Add. Contact 2 e-mail:"]
[]
[null,null,"Center: ",null,null,null,null,null,null,null,"Group Name:"]
[]
[null,null,"Experiment Name:",null,null,null,null,null,null,null,"Official code project:"]
[]
[null,null,"CRG Quotation adressed to (name):",null,null,null,null,null,null,null,"CRG Quotation associated nº:"]
[null,null,null,null,"(email):"]
[null,null,null,null,null,null,null,null,null,null,"TENDER / Minor contract reference:"]
[null,null,"Date:",null,null,null,null,null,null,null,"CRG subproject nº:"]
[]
[null,null,"APPLICATION ",null,null,null,null,null,null,"SEQUENCING DETAILS"]
[]
[null,null,null,"gDNA",null,null,null,null,null,"Million reads / sample:"]
[null,null,null,"gDNA-PCR free"]
[null,null,null,"ChIP ",null,null,null,null,null,"NovaSeq6000 - NEW"]
[null,null,null,"RNAseq - poly A selection",null,null,null,null,null,"Format",null,null,"Flowcell"]
[null,null,null,"RNAseq -  rRNA depletion",null,null,null,null,null,null,"2 x 50      (paired end - 50bp)",null,null,"S2"]
[null,null,null,"RNAseq - Ultra low input - poly A selection",null,null,null,null,null,null,"2 x 150    (paired end - 150bp)"]
[null,null,null,"RNAseq - Ultra low input - rRNA depletion",null,null,null,null,null,"For other run formats and flowcell types, please contact Genomics Unit."]
[null,null,null,"smallRNA - standard selection micro 18-32nt - (using NEB kit)"]
[null,null,null,"Other size selection for small application (nt):",null,null,null,null,null,"NextSeq2000"]
[null,null,null,null,null,null,null,null,null,"Format",null,null,"Flowcell",null,"Cycles kit"]
[null,null,null,"Other application (specify):",null,null,null,null,null,null,"1 x 50    (single read - 50bp)",null,null,"P2",null,50]
[null,null,null,null,null,null,null,null,null,null,"2 x 50    (paired end - 50bp)",null,null,"P3",null,100]
[null,null,null,null,null,null,null,null,null,null,"2 x 100  (paired end - 100bp)",null,null,null,null,200]
[null,null,null,null,null,null,null,null,null,null,"2 x 150  (paired end - 150bp)",null,null,null,null,300]
[null,null,"OBSERVATIONS",null,null,null,null,null,null,null,"Other length*"]
[]
[null,null,null,null,null,null,null,null,null,"NextSeq500"]
[null,null,null,null,null,null,null,null,null,"Format",null,null,"Flowcell",null,"Cycles kit"]
[null,null,null,null,null,null,null,null,null,null,"1 x 75    (single read - 75bp)",null,null,"MID output",null,75]
[null,null,null,null,null,null,null,null,null,null,"2 x 75    (paired end - 75bp)",null,null,"HIGH output",null,150]
[null,null,null,null,null,null,null,null,null,null,"2 x 150  (paired end - 150bp)",null,null,null,null,300]
[null,null,null,null,null,null,null,null,null,null,"Other length*"]
[]
[null,null,null,null,null,null,null,null,null,"MiSeq"]
[null,null,null,null,null,null,null,null,null,null,"2 x 150  (paired end - 150bp)"]
[null,null,null,null,null,null,null,null,null,null,"2 x 300  (paired end - 300bp)"]
[null,null,null,null,null,null,null,null,null,null,"Other length*"]
[]
[null,null,null,null,null,null,null,null,null,"*Other length: you will fill a complete run. Please, specify sequencing format:"]
[null,null,null,null,null,null,null,null,null,null,"Read 1","index i7","index i5","Read 2"]
[null,null,null,null,null,null,null,null,null,"Cycles"]
[]
[null,null,null,null,null,null,null,null,null,null,"Not for sequencing"]
[]
[]
[null,null,"IMPORTANT: Samples, libraries and sequencing data will be kept for 6 months  after service has been completed.  After that, samples and libraries will be discarded if user have not pick them up, and data will be deleted."]
[]
[]
[null,null,"QUALITY CONTROL OF THE SAMPLES"]
[]
[null,null,null,"YES: required to be checked at Genomics Unit",null,null,null,null,null,"QC of RNA samples: Nanodrop and Bioanalyzer / Fragment Analyzer"]
[null,null,null,"NO: done by user (please attach results) and/or not needed (agreed with Genomics Unit)",null,null,null,null,null,"QC of gDNA samples: Qubit and Agarose gel / Tapestation"]
[null,null,null,null,null,null,null,null,null,"QC of ChIP samples: Qubit"]
[null,null,null,"QC observations"]
[]
[]
[]
[null,null,"SAMPLE DETAILS:",null,null,null,null,null,null,null,null,null,null,null,null,null,null,"TYPE OF ERRORS"]
[]
[null,null,"IMPORTANT: Please read carefully \"Guidelines\" tab to fill correctly the required information.  ",null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Duplicate names"]
[]
[null,null,"Names should be alphanumerical, 6 characters maximum, no space, only accepted underscore.",null,null,null,null,null,null,null,null,null,null,null,null,null,null,"More than 6 characters or not accepted character used"]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Plate Name separado"]
[null,null,null,"If samples are in plates, please fill Plate Name. This have to be previously agreed with Unit.",null,null,null,null,null,null,null,null,null,null,null,null,null,"Plate name error, please correct it",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,1,2,3,4,5,6]
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[null,null,null,"Plate Barcode (filled by Genomics Unit)"]
[]
[null,null,null,"NOTE: If you want to copy values from other file, PASTE AS VALUES"]
[]
[null,null,null,"CRG SAMPLE Barcode         (filled by Genomics Unit)","Sample Name                (6 characters,no space, only underscore)","Type of sample     (RNA, DNA, ChIP)","Sample Specie Name                         (Use NCBI Taxonomy)","Volume       (ul)","Conc. (ng/ul)","Nanodrop 260/280","Nanodrop 260/230","Quality Score (RIN / DIN)     RNA or DNA Integrity Number","Observations","Sample Condition","Sample   Replicate","Well plate",null,"Sample Name Error, please correct it",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"well plate (just in case plate filled)",null,null,"Sample Name separado",null,null,null,null,null,null,"Concatenado (incluye\"error\")","encuentra \"error\" y te da la 1º posicion","Comment \"error\" at 1st position"]
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[]
[null,null,null,"NOTE: If you have more than 96 samples, please fill new request form for next samples."]