{"text_format":"1","workbook":"xlsx","n_sheets":2,"sheets":["1. Guidelines","2.Sequencing Request LIBRARIES "],"not_rendered":{"charts":0,"images":10,"macros":1,"external_links":0,"defined_names":2,"comments":0,"pivot_tables":0},"unopened":[],"bounds":{"document_bytes":16777216,"sheet_cells":100000,"rows_first":2000,"rows_last":500,"rows_between":2000,"halvings":0,"cell_chars":16384,"cell_head_chars":4096},"cells_cut":0,"cells_raw":0,"read":"whole","date_system":"1900"}

{"sheet":"1. Guidelines","index":0,"n_rows":136,"n_cols":21,"rows_kept":136,"cut":false,"hidden":false,"merged":[[72,1,73,15],[89,2,90,13],[95,2,97,14],[99,2,101,15],[117,14,117,16],[84,2,85,18],[91,2,91,12],[109,2,109,13],[67,5,67,9],[67,1,67,2],[68,5,68,9],[69,5,69,9],[70,5,70,9],[68,1,68,2],[69,1,69,2],[70,1,70,2],[67,3,67,4],[68,3,68,4],[69,3,69,4],[70,3,70,4],[66,1,66,2],[66,3,66,4],[66,5,66,9],[5,5,5,13],[6,5,6,13],[5,1,5,4],[6,1,6,4],[65,5,65,9],[47,1,47,17],[65,1,65,2],[65,3,65,4]]}
[]
[null,"HOW TO ANALYZE LIBRARIES TO SEQUENCE AT GENOMICS UNIT"]
[]
[null,"Define library region"]
[null,"Whole library has to be included into the region from 150 - 1200bp, independently of the type of libray. DO NOT include:"]
[null,"Primer-dimer:",null,null,null,"Peak around 125-135bp (check example below). If this peak represents more than 5% of the whole library, it should be removed by user. AMPure beads purification would be the method of choice. Using a 0.9x bead ratio is usually suficient. Ask Genomics Unit in case of doubts."]
[null,"Fragment sizes higher than 1200bp: ",null,null,null,"Sequencer is not capable to form clusters with fragments higher than 1200bp."]
[]
[null,"Examples types of libraries"]
[null,"RNAseq",null,null,null,null,null,"ChIPseq",null,null,null,null,null,"ATACseq",null,null,null,null,null,"gDNA"]
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[]
[]
[]
[]
[]
[]
[]
[]
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[]
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[]
[null,"DO NOT include regions in red. In this case, 130bp peak has to be removed before sequencing."]
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[null,"HOW TO FILL SEQUENCING REQUEST LIBRARIES"]
[]
[null,"Remember that you have to fill 1 Request form per application. "]
[null,"* Mandatory fields"]
[]
[null,"CONTACT and SUBPROJECT DETAILS"]
["*","Main User: User responsible for the samples and the project. Please specify full name and contact e-mail."]
["*","PI name: PI responsible for the project. Please specify full name and contact e-mail."]
[null,"Additional contact 1 or 2: Any person that needs to receive information about the project and the final data. Please specify full name and contact e-mail."]
["*","Center: your institution."]
[null,"Group name: your group name inside your institution."]
[null,"Experiment name: name that you give to your experiment."]
[null,"Official code project: example FIS-089932."]
["*","CRG Quotation addressed to (name & email): Person to whom Genomics Unit have to address the quotation, usually PI or other collaborator who will pay the project."]
["*","CRG Quotation associated nº: CRG quotation number for the requested service. If you have not requested it in advance and you do not have it, please, leave it in blank."]
[]
[null,"APPLICATION"]
["*","Application: Please, select the desired application. If you need other application not listed, please specify it in the corresponding field and contact Genomincs Unit."]
[]
[null,"LIBRARY PREPARATION KIT"]
[null,"Please, indicate full name library preparation kit, reference and company."]
[]
[null,"SEQUENCING DETAILS"]
[null,"NEW - New sequencer available at the Genomics Unit, NovaSeq 6000."]
["*","Million reads / sample: nº of millions reads that you need for each sample."]
["*","Sequencing format: Please, indicate your desire sequencing format. Here you have a brief list of sequencer specifications:"]
[null,"Sequencer",null,"Flowcell / kit type",null,"Million reads per flowcell (Expected) "]
[null,"NovaSeq6000",null,"S2",null,3000]
[null,"NextSeq2000",null,"P2",null,400]
[null,"NextSeq2000",null,"P3",null,1200]
[null,"NextSeq500",null,"Mid Output",null,130]
[null,"NextSeq500",null,"High Output",null,400]
[]
[null,"Other length: If you need a run with a sequencing format not listed in the request, is mandatory to fill a complete run, take into account specifications for each sequencer. After that, please indicate the sequencing format (cycles for Read1 - index i7 - index i5 - Read2)."]
[]
[null,"In case of any doubts, contact Genomics Unit."]
["*","Not for sequencing: You only need library preparation, not sequencing at the Unit."]
[null,"Select one option: Sequencing format or Not for sequencing"]
[]
[null,"OBSERVATIONS"]
[null,"Please, indicate any important/special issue with your project."]
[]
[null,"QUALITY CONTROL OF THE LIBRARIES"]
["*","YES / NO: select one option. It refers to check library profiles before sequencing with Bioanalyzer or Fragment Analyzer, qPCR is mandatory before sequencing and is done at Genomics Unit."]
[null,null,"YES: it is need to analyze library profiles at Genomics Unit."]
[null,null,"NO: it has been done by user and agreeded with Genomics Unit not to repeat it. It is mandatory to fill the corresponding information in the table Pool information or Individual libraries and attach the results properly analyzed (you can check How to analyze libraries to sequence at Genomics Unit)."]
[]
[]
[null,"POOL / LIBRARY DETAILS"]
[null,"Pool Information (just fill one row / pool)"]
[null,"*","Pool Name: Use the same name as in the tubes. Names should be alphanumerical, 6 characters maximum, no space, only accepted underscore. These fields have restrictions, in case of error it is indicated in the next columns, please correct it."]
[]
[null,"**","Pool molarity (nM): Value from the defined region. Mandatory nM (nmol/l), be careful when the assay is a High Sensitivity, as this value is given as pM (pmol/l).  If you have run a dilution of the library that you send, make sure to take into account the dilution factor when calculate the final molarity (see example below). Maximum 2 decimals."]
[null,"**","Pool size (bp): Average size of the defined region."]
[null,"*","Pool volume: Volume of pool that you will send. Minimum 10ul."]
[null,"Individual Libraries Information"]
[null,"*","Library Name: Use the same name as in the tubes. Names should be alphanumerical, 6 characters maximum, no space, only accepted underscore. These fields have restrictions, in case of error it is indicated in the next columns, please correct it. If it is a part of a pool, this is the name of the different libraries inside the pool and we will use it to send you the sequencing data. "]
[]
[]
[null,"*","Sample Specie: Use NCBI Taxonomy name, not taxonomy ID. Example: Homo sapiens, not 9606."]
[null,"*","Sequence Multiplex Index P7 and P5: Be sure no spaces are introduced before or after sequence. If a sequence or combination of sequences are duplicated, cells appears in orange, please correct the error. This is very important if libraries will be sequenced together on the same lane. Double-check the sequence is NOT the reverse complement of the index (example below)."]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[null,"**","Library molarity (nM): Value from the defined region. Mandatory nM (nmol/l), be careful when the assay is a High Sensitivity, as this value is given as pM (pmol/l). If you have run a dilution of the library that you send, make sure to take into account the dilution factor when calculate the final molarity (see example below). Maximum 2 decimals. If lbrary is a part of a pool do not fill it."]
[null,"**","Library size (bp): Average size of the defined region.  If lbrary is a part of a pool do not fill it."]
[null,"*","Library volume: Volume of library that you will send. Minimum 10ul."]
[null,null,"**These fields are mandatory if pool/library profile is already done by user and it have been agreed with Genomics Unit of not to repeat them."]
[]
[null,"If you paste values from other files, please PASTE AS VALUES"]
[null,"Once all information is correctly filled without errors, you can send Sequencing Request form to ngs@crg.eu."]
[]
[null,"Example how to fill Sequencing Request (Pool and Libraries Information)"]
[]
[]
[]
[]
[]
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[]
[]
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[]
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[]
[]
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[null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Correct Sample Name Format"]

{"sheet":"2.Sequencing Request LIBRARIES ","index":1,"n_rows":187,"n_cols":147,"rows_kept":187,"cut":false,"hidden":false,"merged":[[14,13,14,17],[16,13,16,17],[21,14,21,17],[18,14,18,17],[6,4,6,8],[8,13,8,17],[6,13,6,17],[10,13,10,17],[12,13,12,17],[12,5,12,8],[10,5,10,8],[8,4,8,8],[16,4,16,8],[14,4,14,8],[21,4,21,5],[19,5,19,8],[38,2,42,8],[18,5,18,8],[33,4,33,7],[46,2,59,8],[62,2,63,17],[86,3,86,8],[87,19,88,19],[78,2,79,17],[72,3,73,17],[80,2,80,17],[87,8,88,8],[87,9,88,9],[87,10,88,10],[87,11,88,11],[87,14,88,14],[87,6,88,6],[87,3,88,3],[87,4,88,4],[87,5,88,5],[87,7,88,7],[86,9,86,17],[87,137,88,137],[86,91,87,91],[86,92,87,92],[87,95,88,95],[86,83,87,88],[86,90,87,90],[86,132,87,132],[86,133,87,133],[86,125,87,130],[87,15,88,15],[76,30,77,35],[81,30,81,32],[86,134,87,134],[87,16,88,16],[87,17,88,17],[86,32,87,35],[87,18,88,18],[86,21,87,23],[79,21,80,23],[83,21,84,23],[77,21,78,23],[81,21,82,23]]}
[]
[]
[null,null,"SEQUENCING REQUEST FORM (LIBRARIES)",null,null,null,null,null,null,null,null,null,null,null,"GENOMICS UNIT - CRG Core Facilities"]
[]
[null,null,"CONTACT and SUBPROJECT DETAILS"]
[]
[null,null,"Main User Name:",null,null,null,null,null,null,null,null,"Main User contact e-mail: "]
[]
[null,null,"PI name:",null,null,null,null,null,null,null,null,"PI contact e-mail:"]
[]
[null,null,null,null,"Additional contact 1:",null,null,null,null,null,null,null,"Add. Contact 1 e-mail:"]
[]
[null,null,null,null,"Additional contact 2:",null,null,null,null,null,null,null,"Add. Contact 2 e-mail:"]
[]
[null,null,"Center: ",null,null,null,null,null,null,null,null,null,"Group Name:"]
[]
[null,null,"Experiment Name:",null,null,null,null,null,null,null,null,null,"Official code project:"]
[]
[null,null,"CRG Quotation addressed to (name):",null,null,null,null,null,null,null,null,null,null,"CRG Quotation associated nº:"]
[null,null,null,null,"       (email):"]
[]
[null,null,"Date:",null,null,null,null,null,null,null,null,null,null,"CRG subproject nº:"]
[]
[]
[]
[null,null,"APPLICATION ",null,null,null,null,null,null,null,null,"SEQUENCING DETAILS"]
[]
[null,null,null,"gDNA",null,null,null,null,null,null,null,"Million reads / sample:"]
[null,null,null,"gDNA-PCR free"]
[null,null,null,"ChIP ",null,null,null,null,null,null,null,"NovaSeq6000 - NEW"]
[null,null,null,"RNAseq - poly A selection",null,null,null,null,null,null,null,"Format",null,null,"Flowcell"]
[null,null,null,"RNAseq -  rRNA depletion",null,null,null,null,null,null,null,null,"2 x 50    (paired end - 50bp)",null,null,"S2"]
[null,null,null,"smallRNA",null,null,null,null,null,null,null,null,"2 x 150   (paired end - 150bp)"]
[null,null,null,"Other (specify):",null,null,null,null,null,null,null,"For other run formats and flowcells types, please contact Genomics Unit."]
[]
[null,null,null,null,null,null,null,null,null,null,null,"NextSeq2000"]
[null,null,"LIBRARY PREPARATION KIT",null,null,null,null,null,null,null,null,"Format",null,null,"Flowcell",null,"Cycles kit"]
[null,null,null,null,null,null,null,null,null,null,null,null,"1 x 50    (single read - 50bp)",null,null,"P2",null,50]
[null,null,null,null,null,null,null,null,null,null,null,null,"2 x 50    (paired end - 50bp)",null,null,"P3",null,100]
[null,null,null,null,null,null,null,null,null,null,null,null,"2 x 100  (paired end - 100bp)",null,null,null,null,200]
[null,null,null,null,null,null,null,null,null,null,null,null,"2 x 150  (paired end - 150bp)",null,null,null,null,300]
[null,null,null,null,null,null,null,null,null,null,null,null,"Other length*"]
[]
[null,null,null,null,null,null,null,null,null,null,null,"NextSeq500"]
[null,null,"OBSERVATIONS",null,null,null,null,null,null,null,null,"Format",null,null,"Flowcell",null,"Cycles kit"]
[null,null,null,null,null,null,null,null,null,null,null,null,"1 x 75    (single read - 75bp)",null,null,"MID output",null,75]
[null,null,null,null,null,null,null,null,null,null,null,null,"2 x 75    (paired end - 75bp)",null,null,"HIGH output",null,150]
[null,null,null,null,null,null,null,null,null,null,null,null,"2 x 150  (paired end - 150bp)",null,null,null,null,300]
[null,null,null,null,null,null,null,null,null,null,null,null,"Other length*"]
[]
[null,null,null,null,null,null,null,null,null,null,null,"MiSeq"]
[null,null,null,null,null,null,null,null,null,null,null,null,"2 x 150  (paired end - 150bp)"]
[null,null,null,null,null,null,null,null,null,null,null,null,"2 x 300  (paired end - 300bp)"]
[null,null,null,null,null,null,null,null,null,null,null,null,"Other length*"]
[]
[null,null,null,null,null,null,null,null,null,null,null,"*Other length: you will fill a complete run. Please, specify sequencing format:"]
[null,null,null,null,null,null,null,null,null,null,null,null,"Read 1","index i7","index i5","Read 2"]
[null,null,null,null,null,null,null,null,null,null,null,"Cycles"]
[]
[null,null,null,null,null,null,null,null,null,null,null,null,"Not for sequencing"]
[]
[]
[null,null,"IMPORTANT: Samples, libraries and sequencing data will be kept for 6 months  after service has been completed.  After that, samples and libraries will be discarded if user have not pick them up, and data will be deleted."]
[]
[]
[null,null,"QUALITY CONTROL OF LIBRARIES"]
[]
[null,null,null,"YES: required to be checked at the Unit",null,null,null,null,null,null,"QC of libraries: Bioanalyzer / Fragment Analyzer and qPCR"]
[null,null,null,"NO: done by user and agreed with Genomics Unit (please attach results) "]
[]
[]
[null,null,null,"QC observations"]
[]
[]
[]
[]
[null,null,"POOL / LIBRARY DETAILS",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"LIMS Upload LIBRARIES"]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"TYPE OF ERRORS"]
[null,null,"IMPORTANT: Please read carefully \"Guidelines\" tab to analyze and fill correctly the required information.                                                                                                                                                            "]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Duplicate names"]
[null,null,"Names should be alphanumerical, 6 characters maximum, no space, only accepted underscore."]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Duplicate index sequence. Please, correct it, very important if libraries will be sequence on same lane.",null,null,null,null,null,null,null,null,"Automatic cells. Do not modify"]
[]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"More than 6 characters or not accepted character used"]
[]
[]
[null,null,null,"POOL INFORMATION (fill just one row / pool)",null,null,null,null,null,"INDIVIDUAL LIBRARIES INFORMATION",null,null,null,null,null,null,null,null,null,null,null,"In case of error, please correct names affected",null,null,null,null,null,null,null,null,null,null,"Copy and Paste (column by column) into                                              \"Edit Selected **Libraries CRG\" LIMS",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Pool Name separado",null,null,null,null,null,null,"Concatenado (incluye\"error\")","encuentra \"error\" y te da la 1º posicion","Comment \"error\" at 1st position",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Library Name separado",null,null,null,null,null,null,"Concatenado (incluye\"error\")","encuentra \"error\" y te da la 1º posicion","Comment \"error\" at 1st position"]
[null,null,null,"CRG POOL Barcode      (filled by Genomics Unit)","Pool Name                (6 characters,no space, only underscore)","Pool Molarity (nM)","Pool          Average Size          (bp)","Pool Volume       (ul)","Pool box CRG  (filled by Genomics Unit)","CRG LIBRARY Barcode         (filled by Genomics Unit)","Library Name                                      (6 characters,no space, only underscore)","Sample Specie (Use NCBI Taxonomy)","Sequence","Sequence","Molarity (nM)              Do not fill in case of pool","Average Size          (bp)              Do not fill in case of pool","Volume              (ul)                         Do not fill in case of pool","Library box CRG            (filled by Genomics Unit)","Sample Condition","Sample Replicate",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"duplicate names","final comment","Formatting in red library name and library error",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"duplicate names","final comment","Formatting in red library name and library error"]
[null,null,null,null,null,null,null,null,null,null,null,null,"Multiplex Index P7","Multiplex Index P5",null,null,null,null,null,null,null,"Pool Name Error","Library Name Error","Multiplex index error",null,null,null,null,null,null,"Library barcode (sorted Z to A)","Library name","Library molarity (nM)","Library size     (bp)","Library volume (ul)","Library box number",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,1,2,3,4,5,6,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,1,2,3,4,5,6,null,null,null,null,null,null,null,"Duplicados P7","Duplicados P5",null,null,"Comprobar combinación","duplicados combinación","Comentario de la combinación","Final comment","Formatting in orange duplicate barcode sequence"]
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