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PRBB-CRG Sessions David Garflied

PRBB-CRG Sessions David Garflied

05/07/2019
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PRBB-CRG Sessions David Garflied

MARIE CURIE

05/07/201912:00MARIE CURIEPRBB-CRG SessionsDavid GarfliedHumboldt University Berlin, DE"Characterising the functional consequences of non-coding mutations using single-cell ATAC-Seq in non-human model systems"Host: Arnau Sebé-PedrosAbstract:Many of the mutations that define us – the ones that make us tall vs. short or healthy vs. sick and even the mutations that make us human, are found distributed across the non-coding, regulatory genome. Identifying and characterizing these mutations, however is challenging; unlike coding loci, the locations and function of DNA regulatory elements cannot typically be determined by DNA sequence alone. Regulatory elements are also highly context specific, with many elements showing activity and accessibility to protein binding in a subset of tissues or developmental stages. Single-cell chromatin profiling provides on solution to these issues by allowing researchers to identify protein-bound regulatory elements across multiple tissues and developmental stages in a single experiment.
In this talk, I will present an overview of how my lab is using single-cell chromatin profiling in non-mammalian model systems (Drosophila and sea urchins) to better understand the regulatory elements active during embryogenesis and how mutations impact their function. I will focus specifically on two questions: 1) How do tissue/stage-specific regulatory architectures influence the functional consequences of mutations? 2) What are regulatory elements specified during the earliest stages of embryogenesis?