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PRBB-CRG Sessions Bas van Steensel

PRBB-CRG Sessions Bas van SteenselPRBB-CRG Sessions Bas van Steensel

19/01/2024
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PRBB-CRG Sessions Bas van Steensel

MARIE CURIE

19/01/202412:00MARIE CURIEPRBB-CRG SessionsBas van SteenselNetherlands Cancer Institute and Oncode Institute"Scrambling the genome to study gene regulation"Host: Lars VeltenAbstract:In mammalian genomes, the regulatory elements that control a promoter are often scattered over tens to hundreds of kb. How such apparently haphazard linear arrangements can result in specific gene regulation is a major puzzle. To unravel the underlying logic, we have developed a scalable technology to transplant selected DNA elements to hundreds of alternative positions within a ~2 Mb region, and track the functional consequences. We have used this methodology to systematically dissect the functional architecture of a large genomic locus in mouse cells. Furthermore, we have combined the tool with Cre-LoxP recombination to generate systematic series of megabase-sized deletions and inversions, and used this approach to dissect how lamina-associated domains are tethered to the nuclear lamina. Our technology will help to elucidate how the ordering and spacing of regulatory elements along the genome contributes to optimal gene regulation.