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PRBB Computational Genomics Seminars Rory Johnson

PRBB Computational Genomics Seminars Rory JohnsonPRBB Computational Genomics Seminars Rory Johnson

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PRBB Computational Genomics Seminars Rory Johnson


19/05/202212:00R_473.10_AULAPRBB Computational Genomics SeminarsRory JohnsonUniversity College Dublin, Ireland / Institute of Bioorganic Chemistry, PolandCRISPR-powered development of (long noncoding) RNA therapeutics in oncologyHost: Garrido Enamorado, RominaAbstract:Zoom link:

Rory Johnson
School of Biology and Environmental Science, University College Dublin, Ireland / Department of Medical Oncology, Bern University Hospital, Switzerland
"CRISPR-powered development of (long noncoding) RNA therapeutics in oncology"

Long noncoding RNAs represent a vast and unexplored space of potential disease genes, yet technical and scientific barriers have hindered their exploitation for RNA therapeutics. Twin emerging technologies of CRISPR-Cas9 and antisense oligonucleotides (ASO) now offer the means to both identify and drug disease-promoting lncRNAs for a range of unmet medical needs. Amongst the greatest cancer killers is KRAS-mutated non-small cell lung cancer (NSCLC), where mortality results from uncontrolled cell proliferation, acquired resistance to chemotherapy, and metastasis. In this presentation, I will introduce my team’s development of CRISPR-based lncRNA screening pipeline, and its application to developing promising pre-clinical ASO therapies for NSCLC

Barbara Uszczynska
Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland
"Profiling subcellular localization of nuclear-encoded mitochondrial gene products in zebrafish"

Most mitochondrial proteins are encoded by nuclear genes, synthesised in the cytosol and targeted into the organelle. Therefore, for decades the post-translational import was considered the main route of transporting proteins into mitochondria. Although the machineries and mechanisms of mitochondrial import have been well-described, the cytosolic stage of this process is not fully understood. At the same time, evidence of alternative routes has been debated for a while. Numerous mRNAs that encode mitochondrial proteins were found either on the mitochondrial surface or in close proximity in both yeast. Moreover, they have been shown to be active templates for protein synthesis, suggesting that localised translation can be also coupled with the direct transport of synthesised proteins into mitochondria. This study for the first time profiles subcellular localization of mitochondrial gene products in vertebrates using zebrafish (Danio rerio) as a model organism