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Cell and Developmental Biology Programme Emanuele Leoncini

Cell and Developmental Biology Programme Emanuele Leoncini

17/12/2018
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Cell and Developmental Biology Programme Emanuele Leoncini

CHARLES DARWIN

17/12/201815:00CHARLES DARWINCell and Developmental Biology ProgrammeEmanuele LeonciniHarvard University"Following single-cell physiology of bacteria through booms and busts"Host: Böke, ElvanAbstract:As microbes deplete local resources they undergo great changes in size, morphology, growth conditions and gene expression. Even in a clonal population, responses vary from cell to cell making it necessary to track individual cells along the bacterial growth curve. We developed a microfluidic platform for tracking >105 cell lineages in parallel even in dense and growing cultures with single-cell resolution under controlled conditions. We used this platform to study growth regimes along the growth curve, and to analyze strategies on how individual cells enter/exit from stationary phase.